Product Description

MicroRNA, a class of non-coding Rnas with a length of about 22 nt, is widely concerned and plays a very important role in regulating gene expression in plants and animals. This kit uses SYBR Green I chimeric fluorescence method for quantitative detection of miRNA fluorescence. The 2×Hieff^TM miRNA Universal qPCR SYBR Master Mix is a new generation of premix fluorescent quantitative PCR detection reagent specially developed for the quantitative detection of miRNA. It contains special ROX Passive Reference Dye. Suitable for all qPCR instruments, there is no need to adjust the concentration of ROX on different instruments, just add primers and templates in the preparation of reaction system can be amplified. The DNA Polymerase uses chemically modified heat-activated Polymerase, combined with a special Buffer system, to make the reaction more specific, more sensitive, and can be accurately quantified in a wider range.

This product is recommended for use with our Hifair^TM miRNA 1st Strand cDNA Synthesis Kit (plus A method) (Cat#11148) to obtain optimal experimental results.

Shipping and Storage

The product is shipped with ice packs and can be stored at -20°C for 1 year.

Reagents and Consumables to Be Prepared by Yourself

1) No nuclease contamination of the head and centrifuge tube.

2) Forward Primer for PCR.

Forward Primer Design Principles

Based on mature miRNA sequences, U is usually replaced with T. The Tm value is recommended to be around 65℃. Several G or C bases can be appropriately added to the 5 'end of the primer to increase Tm value, or several bases can be appropriately removed from the 5' or 3 'end of the primer to reduce Tm value, and the introduction of secondary structure should be avoided.

Cautions

1. Before use, fully melt the frozen components and gently mix them before use.

2. Keep the experimental area clean and ensure all the supplies is RNase free.

3. Please avoid repeated freezing and thawing and avoid strong light when preparing.

4. For your safety and health, please wear lab coats and disposable gloves for operation.

5. This product is for research use ONLY！