Product Description

DNA Polymerase I, in the presence of template and primer (DNA or RNA), uses dNTP as a substrate to synthesize DNA complementary to the template in the 5'→3' direction. The molecular weight of this enzyme is about 109,000 Da, and it has double-strand-specific 5'→3' exonuclease activity and single-strand-specific 3'→5' exonuclease activity. This enzyme can be used with DNase I (Cat#10607) for nick translation reaction and with RNase H (Cat#12906) to synthesize the second strand of cDNA.

Product Components

Components		12903ES60	12903ES76	12903ES92
12903-A	DNA polymerase I	50 μL	250 μL	1 mL
12903-B	10× DNA Polymerase I Reaction Buffer	1 mL	1 × 5 mL	4 × 5 mL

*10× DNA Polymerase I Reaction Buffer: 500 mM Tris-HCl (pH 7.5 at 25 °C), 100 mM MgCl₂, 10 mM DTT.

Shipping and Storage

The product is shipped with dry ice and can be stored at -20°C for two years.

Cautions

1. For your safety and health, please wear lab coats and disposable gloves for operation.

2. This product is for research use ONLY!

Applications

Labeling 3'-termini of nucleic acids

Nick translation of DNA

Second strand synthesis of cDNA