Murine RNase inhibitor (40 U/μL)

Description

Murine RNase Inhibitor is purified from a recombinant strain of E. coli in a soluble form. It specifically inhibits the activity of RNases A, B and C through binding noncovalently in a 1:1 ratio with high affinity. Recombinant murine RNase inhibitor does NOT contain 2 oxidation-sensitive cysteine which are contained in human-origin RNase inhibitor. Therefore, murine RNase inhibitor has high anti-oxidation activity and is more stable for low DTT experiments (< 1mmol/L). This product is validated for its compatibility with HifairTM Ⅱ Reverse Transcriptase (Cat#11110) and various DNA Polymerases. Murine RNase inhibitor is ideal for high-DTT-sensitive experiments, such as RT-PCR. The product can be used in cDNA Synthesis, polysome isolation and In vitro transcription/translation.

Features

• Performs under a wide range of reaction conditions
• Protects RNA from degradation at temperatures up to 55°C

Specifications

Concentration	40 U/μL
Product Type	RNase Inhibitor
Unit Definition	The required amount of RNase Inhibitor to inhibit 50% activity of 5-ng RNase A is defined as one unit. The activity of RNase A is measured by hydrolyzing of cyclic 2', 3'-CMP to generate 3'-CMP.

Components

Components No.	Name	10603ES05 (2 KU)	10603ES10 (10 KU)	10603ES20 (20 KU)	10603ES60 (100 KU)	10603ES94 (20,000 KU)
10603	Murine RNase Inhibitor (40 U/µL)	50 μL	250 μL	500 μL	2.5 mL	500 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -20°C for 2 years.