Product Description

RT-LAMP Kit (UDG plus) contains Bst plus DNA Polymerase, thermotolerant Hifair™ Ⅲ Reverse Transcriptase, Murine RNase Inhibitor (MRI) and UDGase. This product is suitable for LAMP reaction with RNA as a template. The UDG in this kit can effectively prevent aerosol contamination.

Package Information

Component Number	Components	Cat#/Size
		13762ES60 (100 T)	13762ES80 (1,000 T)
13762-A	Bst Buffer	600 µL	6 mL
13762-B	Bst Enzyme Mix (UDG plus)	100 µL	1 mL

Shipping and Storage

1. Shipping

The product is shipped with dry ice.

2. Storage

Part 1: 13762-A should be protected from light and can be stored at -20°C for 6 months.

Part 2: 13762-B can be stored at -20°C for 6 months.     

Cautions

For your safety and health, please wear lab coats and disposable gloves for operation. 

Preparation of the reaction system

Components	Volume (μL)	Final Concentration
RNase free ddH2O	to 25	-
Bst Buffer	6	1×
10× Primers	2.5	-
Bst Enzyme Mix (UDG plus)	1	-
Template	10 ng-1 μg	-

Note: 

1. Bst Buffer contains dN (U) TP and SYTO-9 etc., and the concentration of Mg2+ in the 1× Bst Buffer is 8mM.

2. Bst Enzyme Mix (UDG plus) contains a Bst DNA Polymerase, thermotolerant Hifair® Ⅲ Reverse Transcriptase, Murine RNase Inhibitor (MRI), and UDGase.

3. Recommend 10×LAMP Primer Mix: 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/Loop B. Or, the ratio of other best primer groups can be tested.

Reaction Condition

The components were added, mixed, centrifugated, and reacted, incubated at 37°C for 2-5min, then incubated at a constant temperature of 60-65°C for 30-60min and deactivated at 85°C for 5min in a fluorescence quantitative PCR instrument. The reaction type should be SYBR Green Reagents.