Description

Hieff™ Double-Block Anti-Taq DNA Polymerase Antibody is a double-blocking Taq DNA Polymerase monoclonal antibody for hot start PCR. It can inhibit the activity of 5 '- 3' polymerase and 5 '- 3' exonuclease after binding to Taq DNA Polymerase, which can effectively inhibit the nonspecific annealing of primers and the nonspecific amplification caused by primer dimer at low temperatures. In addition, the product can effectively prevent probe degradation. HieffTM Double-Block anti-Taq DNA Polymerase Antibody is denatured in the initial DNA denaturation step of PCR reaction, through which the activity of DNA polymerase is restored to achieve the effect of hot start PCR. It can be used under the condition of routine PCR reaction without special inactivation of antibodies.

Applications

• The concentration of this product is 5 mg/mL. 1 μL antibody could block the activity of 20-50 U Taq DNA polymerase. It is recommended to mix the antibody and the Taq DNA polymerase well and incubate at room temperature for 20 min prior to use. The blocking efficiency of 5 '- 3' polymerase and 5 '- 3' exonuclease activity is over 90%.

Specifications

Classification	Monoclonal
Type	Antibody
Antigen	Taq DNA Polymerase
Form	Liquid

Components

Components No.	Name	31303ES60/31303ES80	31303ES90/31303ES92	31303ES93/31303ES94
31303-A	Hieff™ Double-Block Anti-Taq DNA Polymerase Antibody	100 µg/1 mg	5 mg/10 mg	100 mg/1000 mg

Shipping and Storage

The product is shipped with ice packs and can be stored at -25°C~-15℃ for 2 years.