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0Hifair™ Ⅲ Reverse Transcriptase
Product Description
Hifair™ Ⅲ Reverse Transcriptase is an updated version of Hifair™ Ⅱ Reverse Transcriptase obtained through genetic engineering technology. It has higher cDNA synthesis ability and speed, thermal stability and reaction temperature limit (up to 60°C) than Hifair™ Ⅱ Reverse Transcriptase. The synthesized cDNA product is up to 19.8 kb. Hifair™ Ⅲ Reverse Transcriptase enhances the affinity of the templates and is suitable for reverse transcription of RNA templates with complex secondary structure or low copy genes.
Application
Full-length cDNA library construction; End-point PCR; Real-time PCR.
Unit Definition
One unit is defined as the amount of enzyme required for incorporating 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using Oligo(dT) as primers.
Shipping and Storage
The product is shipped with ice packs and can be stored at -20°C for 1 year.
Product Notes
1. Keep the experimental area clean and use RNase-free supplies.
2. All operations should be carried out on ice to prevent RNA degradation.
3. High-quality RNA samples are recommended for efficient reverse transcription.
4. For your safety and health, please wear lab coats and disposable gloves for operation.
5. This product is for research use ONLY!
[1] Wang J, Song J, Song G, et al. Acetyl-L-carnitine improves erectile function in bilateral cavernous nerve injury rats via promoting cavernous nerve regeneration. Andrology. 2022;10(5):984-996. doi:10.1111/andr.13187(IF:3.842)
[2] Wang J, Jia Z, Dang H, Zou J. Meteorin-like/Meteorin-β upregulates proinflammatory cytokines via NF-κB pathway in grass carp Ctenopharyngodon idella. Dev Comp Immunol. 2022;127:104289. doi:10.1016/j.dci.2021.104289(IF:3.636)
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