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Hieff NGS™ DNA Selection Beads

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Details:

Description

Hieff NGS™ DNA Selection Beads are prepared based on the SPRI (Solid Phase Reverse Immobilization) principle and are applicable for DNA purification and size selection during the preparation of next-generation sequencing (NGS) libraries. Hieff NGS™ DNA Selection Beads are compatible with various DNA and RNA library prep kits.     

Features   

  • High recovery, for nucleic acid fragments between 200bp to 20kb, the recovery rate reaches 95%;
  • Effectively remove redundant dNTPs, primers, primer dimers, salts, and other impurities
  • Can purify dsDNA or ssDNA
  • Precise, controllable, and highly repetitive fragment selection

Applications

  • DNA clean up (above 200bp)
  • DNA/RNA library construction
  • Purification of PCR products
  • Purification of enzyme digestion product
  • DNA size selection

Specifications

Product Line DNA clean and selection Beads
Starting Material DNA
Compatibility DNA
Isolation Technology Magnetic Bead
Final Product Type DNA
For Use With (Application) DNA celan up, DNA size slection

Components

Components No. Name 12601ES08 12601ES56 12601ES75
12601 Hieff NGS™ DNA Selection Beads 5 mL 60 mL 450 mL

Shipping and Storage

The beads are shipping with ice packs and can be stored at 2°C-8°C for one year.

Figures

  • DNA size selection

The calf thymus DNA was fragmented by sonication to prepare a fragment of 100-1,000 bp, and two rounds of size selection were performed according to Table 1. The results were analyzed using Agilent 2100 Bioanalyzer (Figure 1).

Table 1. Recommended condition for DNA size selection

Length of DNA fragment 250-350 bp 320-420 bp 450-550 bp 550-700 bp 700-900 bp 800-1,000 bp
Ratio of Beads: DNA for the 1st Round 0.80× 0.70× 0.60× 0.55× 0.50× 0.45×
Ratio of Beads: DNA for the 1st Round 0.20× 0.20× 0.20× 0.15× 0.15× 0.15×

Note: "×" in the table indicates the volume of sample DNA. For example, if the insert length of the library is 250 bp and the sample DNA volume is 100 μL, the volume of magnetic beads used in the first round of sorting is 0.80×100 μL=80 μL; the volume of magnetic beads used in the second round of sorting is 0.20× 100 μL=20 μL.

Figure 2. Agilent 2100 high-sensitivity DNA chip electropherogram

  • DNA purification

Table 2. Recommended condition for DNA purification

Beads ratio Recovery CONC. (ng/μL) Recovery Rate
1 2 3 Average
1.8× 37.8 36 36.2 36.67 98.09%
0.8× 33.2 33 32.2 32.80 87.75%
0.6× 28.8 28.8 27.8 28.47 76.15%

Sample: 1kb ladder ; M: 1kb DNA ladder; Input amount: 785ng；Elution volume: 21μL

Figure 2 Agarose gel electrophoresis results

Sample: 1kb ladder; M: 1kb DNA ladder; Input amount 

Documents:
Citations & References:

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[6] Gao X, Yu B, Yu J, et al. Developmental Profiling of Dietary Carbohydrate Digestion in Piglets. Front Microbiol. 2022;13:896660. Published 2022 Apr 29. doi:10.3389/fmicb.2022.896660(IF:5.640)

[7] Li P, Zhang Y, Yan F, Zhou X. Characteristics of a Bacteriophage, vB_Kox_ZX8, Isolated From Clinical Klebsiella oxytoca and Its Therapeutic Effect on Mice Bacteremia. Front Microbiol. 2021;12:763136. Published 2021 Dec 3. doi:10.3389/fmicb.2021.763136(IF:5.640)

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