This product consists of 9 highly purified and prestained proteins ranging in molecular weight from 2.7 kDa to 40kDa (2.7,4.2,6.5,10,15,20,25,30,40 kDa), among them, 40 kDa is orange band, 10 kDa is green band. The labeled apparent molecular weight was calibrated by the molecular weight Marker of standard non-prestained proteins. Using this product, the state of protein electrophoresis and the effect of membrane transfer can be dynamically observed. After SDS-PAGE electrophoresis, the color bands were transferred to PVDF membrane and NC membrane. This product is conveniently packaged and is a ready-to-use product, do not heat, dilute or add reducing agents! After the prestained protein is combined with the dye, in different buffer systems, there is displacement. this product is only for reference when judging the molecular weight of the target protein.
Features
Applications
Product Type Specs | Protein Ladder |
Size Range | 2.7 kDa to 40 kDa |
Number of Markers | 9 |
Molecular Weight | 2.7,4.2,6.5,10,15,20,25,30,40 kDa |
Stain Type | 40 kDa is orange band, 10 kDa is green band, the rest are blue bands |
Storage Buffer | 62.5 mM Tris-H3PO4(pH 7.5), 2 mM EDTA, 2% (W/V) SDS, 33% (W/V) Glycerol, 5 mM DTT, 0.02% (V/V) proclin300 |
Ready to Load | ready-to-use product, do not heat, dilute or add reducing agents |
Components No. | Name | 20344ES72 | 20344ES76 | 20344ES90 |
20344 | GoldBand™ 3-color Low Range Protein Marker (2.7-40 kDa) | 250 μL | 2×250 μL | 10×250 μL |
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. For regular use, it can be placed at 4℃, valid for three months. It is recommended to store in aliquots to avoid repeated freezing and thawing!
Figure 1 18% SDS-PAGE electrophoresis results
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