UCF.ME™ UltraNuclease GMP-grade

Description

UltraNuclease GMP-grade from YEASEN is expressed and purified in Escherichia coli (E. coli) by genetically engineered and prepared under the GMP environments. It can reduce the viscosity of cell supernatant and cell lysate in scientific research, increase protein purification efficiency and enhance protein functional research. The product can also reduce host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein/polysaccharide pharmaceutical manufacturing. Besides, the product can also be applied to prevent the clumping of human peripheral blood mononuclear cells (PBMC) in cell therapy and vaccine development.

UltraNuclease from YEASEN is provided in the form of a sterilized reagent, eluted in buffer (20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerin), with the appearance of a colorless, transparent liquid. This product is produced by GMP process requirements and provided in a liquid form.

Feature

• Wide range of applications: degrade all forms of DNA and RNA 
• High purity and activity: purity ≥ 99%; specific activity ≥ 1.5×10⁶ U/mg
• Strong adaptability: strong tolerance, adapt to a variety of reaction conditions
• Comply with pharmacopeia: no animal origin; no antibiotics; lower endotoxin
• Qualification: product gets the FDA DMF filling which can shorten the customers for new drug application
• GMP-grade manufacturing: strict standards to meet the needs of large-scale use for research to manufacture

Application

• Purification of viral vaccines, viral vectors for vaccine, cell and gene therapy, and oncolytic viruses, removing DNA/RNA from proteins and other biologicals
• Reduction of viscosity caused by nucleic acids
• Sample preparation in electrophoresis and chromatography
• Prevention of cell clumping

Specification

Expression Host	Recombinant E. coli with UltraNuclease gene
Molecular Weight	26.5 kDa
Isoelectric point	6.85
Purity	≥ 99% (SDS-PAGE)
Storage Buffer	20 mM Tris-HCl pH8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerin
Unit Definition	The definition of one Activity unit (U) is the amount of enzyme used to change the absorption value of △A260 by 1.0 in 30 minutes in a 2.625 mL reaction system at 37°C with a pH of 8.0 (equivalent to complete digestion of 37 μg salmon sperm DNA into oligonucleotides).

Components

Components No.	Name	20157ES25 (25 KU)	20157ES60 (100 KU)	20157ES80 (1 MU)	20157ES90 (5 MU)
20157	UltraNuclease GMP-grade (250 U/μL)	100 μL	400 μL	4 mL	20 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. If the product is opened and has been stored at 4℃ for more than a week, we recommend filtering the product to prevent microbial contamination.

Figures

• The effect of various factors on enzyme activity

Figures 1. The effect of temperature and pH (Figures 1A)，cations (Figures 1B)，commonly used surfactants (Figures 1C) on enzyme activity

• Reaction conditions recommend

Table 1A. The optimal reaction conditions for UCF.ME™ UltraNuclease

Factor	Optimum Condition	Effective condition
Mg2+	1-5mM	0-10mM
pH	8-9	6-10
Temperature	37℃	0-42℃
Na+/K+	0-20mM	0-150mM
PO43+	0-10mM	0-100mM
EDTA	0-0.5mM	0-5mM
Triton®X-100	0%-0.4%	0%-1%

Table 1B. The recommended enzyme usage and reaction time under the best conditions (37℃, Mg²⁺ 2mM, pH 8.0)

Amount of nuclease(Final concentration)	Reaction Time
0.25 U/mL	> 10h
2.5 U/mL	＞4h
25 U/mL	30min