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0Hieff NGS™ Complete Adapter Kit for Illumina,Set1
Product Description
Hieff NGSTM Complete Adapter Kits for Illumina® are two special kits for DNA library preparation for Illumina®, each set contains 48 kinds of single Index Complete Adapter. All the reagents provided in the kit are subjected strict quality control and functional verification to ensure the maximum stability and repeatability of the library preparation.
Product Component
|
Cat # |
Component |
Volume |
|
13519ES04 |
DNA Adapter 01-48 |
12 μL each |
|
13519ES16 |
DNA Adapter 01-48 |
48 μL each |
|
13520ES04 |
DNA Adapter 49-96 |
12 μL each |
|
13520ES16 |
DNA Adapter 49-96 |
48 μL each |
Shipping and Storage
Ice bag transportation. All the components can be stored at -20℃ for 18 months.
Notes
1. The concentration of DNA adapter in these kits is 15 μM. The amount of adapter when construct a single library should be adjusted according to the Library preparation Kit protocol.
2. Do not heat the adapters. They should be dissolved slowly at room temperature. It is best to set the laboratory temperature at 20-25℃. Avoid repeated freezing and thawing of the adapters. It is recommended to store them in separate packs. The adapters can be stored at 4℃ for a short time.
3. For your safety and health, please wear experimental clothes and disposable gloves.
4. For research use only.
Sequence
The structure of libraries prepared with Hieff NGSTM Complete Adapter Kit for Illumina® are as follows:
DNA Adapter for Illumina
Universal Adapter：
5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’
Barcode Adapter：
5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACXXXXXXXX1ATCTCGTATGCCGTCTTCTGCTTG-3’
|
DNA Adapter |
Sequence |
DNA Adapter |
Sequence |
|
DNA Adapter 01 |
AACGTGAT |
DNA Adapter 49 |
GATAGACA |
|
DNA Adapter 02 |
AAACATCG |
DNA Adapter 50 |
GCCACATA |
|
DNA Adapter 03 |
ATGCCTAA |
DNA Adapter 51 |
GCGAGTAA |
|
DNA Adapter 04 |
AGTGGTCA |
DNA Adapter 52 |
GCTAACGA |
|
DNA Adapter 05 |
ACCACTGT |
DNA Adapter 53 |
GCTCGGTA |
|
DNA Adapter 06 |
ACATTGGC |
DNA Adapter 54 |
GGAGAACA |
|
DNA Adapter 07 |
CAGATCTG |
DNA Adapter 55 |
GGTGCGAA |
|
DNA Adapter 08 |
CATCAAGT |
DNA Adapter 56 |
GTACGCAA |
|
DNA Adapter 09 |
CGCTGATC |
DNA Adapter 57 |
GTCGTAGA |
|
DNA Adapter 10 |
ACAAGCTA |
DNA Adapter 58 |
GTCTGTCA |
|
DNA Adapter 11 |
CTGTAGCC |
DNA Adapter 59 |
GTGTTCTA |
|
DNA Adapter 12 |
AGTACAAG |
DNA Adapter 60 |
TAGGATGA |
|
DNA Adapter 13 |
AACAACCA |
DNA Adapter 61 |
TATCAGCA |
|
DNA Adapter 14 |
AACCGAGA |
DNA Adapter 62 |
TCCGTCTA |
|
DNA Adapter 15 |
AACGCTTA |
DNA Adapter 63 |
TCTTCACA |
|
DNA Adapter 16 |
AAGACGGA |
DNA Adapter 64 |
TGAAGAGA |
|
DNA Adapter 17 |
AAGGTACA |
DNA Adapter 65 |
TGGAACAA |
|
DNA Adapter 18 |
ACACAGAA |
DNA Adapter 66 |
TGGCTTCA |
|
DNA Adapter 19 |
ACAGCAGA |
DNA Adapter 67 |
TGGTGGTA |
|
DNA Adapter 20 |
ACCTCCAA |
DNA Adapter 68 |
TTCACGCA |
|
DNA Adapter 21 |
ACGCTCGA |
DNA Adapter 69 |
AACTCACC |
|
DNA Adapter 22 |
ACGTATCA |
DNA Adapter 70 |
AAGAGATC |
|
DNA Adapter 23 |
ACTATGCA |
DNA Adapter 71 |
AAGGACAC |
|
DNA Adapter 24 |
AGAGTCAA |
DNA Adapter 72 |
AATCCGTC |
|
DNA Adapter 25 |
AGATCGCA |
DNA Adapter 73 |
AATGTTGC |
|
DNA Adapter 26 |
AGCAGGAA |
DNA Adapter 74 |
ACACGACC |
|
DNA Adapter 27 |
AGTCACTA |
DNA Adapter 75 |
ACAGATTC |
|
DNA Adapter 28 |
ATCCTGTA |
DNA Adapter 76 |
AGATGTAC |
|
DNA Adapter 29 |
ATTGAGGA |
DNA Adapter 77 |
AGCACCTC |
|
DNA Adapter 30 |
CAACCACA |
DNA Adapter 78 |
AGCCATGC |
|
DNA Adapter 31 |
GACTAGTA |
DNA Adapter 79 |
AGGCTAAC |
|
DNA Adapter 32 |
CAATGGAA |
DNA Adapter 80 |
ATAGCGAC |
|
DNA Adapter 33 |
CACTTCGA |
DNA Adapter 81 |
ATCATTCC |
|
DNA Adapter 34 |
CAGCGTTA |
DNA Adapter 82 |
ATTGGCTC |
|
DNA Adapter 35 |
CATACCAA |
DNA Adapter 83 |
CAAGGAGC |
|
DNA Adapter 36 |
CCAGTTCA |
DNA Adapter 843 |
CACCTTAC |
|
DNA Adapter 37 |
CCGAAGTA |
DNA Adapter 85 |
CCATCCTC |
|
DNA Adapter 38 |
CCGTGAGA |
DNA Adapter 86 |
CCGACAAC |
|
DNA Adapter 39 |
CCTCCTGA |
DNA Adapter 87 |
CCTAATCC |
|
DNA Adapter 40 |
CGAACTTA |
DNA Adapter 88 |
CCTCTATC |
|
DNA Adapter 41 |
CGACTGGA |
DNA Adapter 89 |
CGACACAC |
|
DNA Adapter 42 |
CGCATACA |
DNA Adapter 90 |
CGGATTGC |
|
DNA Adapter 43 |
CTCAATGA |
DNA Adapter 91 |
CTAAGGTC |
|
DNA Adapter 44 |
CTGAGCCA |
DNA Adapter 92 |
GAACAGGC |
|
DNA Adapter 45 |
CTGGCATA |
DNA Adapter 93 |
GACAGTGC |
|
DNA Adapter 46 |
GAATCTGA |
DNA Adapter 94 |
GAGTTAGC |
|
DNA Adapter 47 |
CAAGACTA |
DNA Adapter 95 |
GATGAATC |
|
DNA Adapter 48 |
GAGCTGAA |
DNA Adapter 96 |
GCCAAGAC |
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