Description

Hieff NGS^TM Ultima Pro DNA Library Prep Kit for Illumina V2 is a new generation library construction kit specially developed and designed for the Illumina high-throughput sequencing platform. On the basis of the previous generation of library construction kit, this product exhibits higher efficiency in end repair, dA-tailing, and adapter ligation than the previous versions. The high-fidelity enzyme significantly improves the uniformity and fidelity of amplification. The kit is compatible with most DNA sample types, including standard genomic DNA from animals/plants/microorganisms, FFPE samples, cfDNA, and ChIP DNA. Compatible with 100 pg - 1000 ng DNA samples of most types, including cfDNA and FFPE samples.

Features

• Compatible with multiple types of DNA samples：animal and plant gDNA, microbial gDNA, FFPE DNA, cfDNA, ChIPDNA, etc., support the construction of PCR free library
• With an industry-leading library conversion rate of more than 70%
• Proven to provide high-quality libraries and sequencing data.
• Strict batch stability

Applications

• Construction of DNA library for illumina platform
• Suitable for sequencing in tumor, reproductive genetics, genetic disease detection and other fields
• PCR-free library constrction

Specifications

Product Type	Library Preparation Kit
Libraries	Fragment Library
Fragmentation method	Ultrasonic
Input amount	100 pg - 1000 ng
For Use With (Equipment)	Illumina Platforms
Sample Type	gDNA
Sequencing Type	Genome & DNA Sequencing
Product Line	DNA library constrction
For Use With (Application)	NGS DNA library preparation
Quantity	8 /24/96 Reactions

Components

Components No.	Name	12197ES08 (8T）	12197ES24 （24T）	12197ES96 (96T）
12197-A	Endprep Buffer 2.0	100 μL	400 μL	4 mL
12197-B	Endprep Enzyme 2.0	48 μL	144 μL	576 μL
12197-C	Ligation Enhancer 2.0	32 μL	96 μL	384 μL
12197-D	Rapid T4 DNA Ligase 2.0	240 μL	720 μL	3×960 μL
12197-E	CanaceTM Pro Amplification Mix 2.0	40 μL	120 μL	480 μL
12197-F	Primer Mix	200 μL	600 μL	3×800 μL

Shipping and Storage

Dry ice shipping. -15℃ ~ -25℃ storage, valid for one year.

Figures

Figure 1. Comparison of cfDNA library yields

Sample: cfDNA 5ng; Number of PCR cycles: 12 cycles; Method: use different library preparation kits to construction  library, then compare the library yields.

Figure 2. Comparison of hybridized capture libraries of tumor SNV 0.5% and 1% gDNA 

Sample: DNA with a mutation frequency of 0.5% and 1%，50ng each; Number of PCR cycles: 12 cycles; Method: The constructed library was captured with IDT xGen^TM Pan-Cancer Hyb Panel, and the library was sequenced.the library yield, sequencing data quality and gene mutation detection were compared.

Table 1. Quality control data