Product Description

Monkeypox Virus Real Time qPCR Kit (UDG plus) is a PCR kit for the detection of monkeypox virus. Contains monkeypox virus primer probes, using the company's new-generation antibody method hot-start Taq enzyme 2× Mix master mix reagent and positive control, including Mg2+ and dNTP, etc., and adding factors and enhancements that effectively inhibit non-specific PCR amplification The factor of the amplification efficiency of the multiplex qPCR reaction can improve the amplification efficiency of the reaction and promote the efficient amplification of low-concentration templates. This product contains UDG enzymes, which effectively prevent the risk of aerosol contamination.

Product Components

Component		13863ES25(25 T)	13863ES60(100 T)	13863ES80(1,000T)
13863-A	2×TaqMan qPCR Mix	313 μL	1.25 mL	12.5 mL
13863-B	Prime&Probe Mix	25 μL	100 μL	1 mL
13863-C	MPV Negative Control	125 μL	500 μL	5 mL
13863-D	MPV Positive Control	125 μL	500 μL	5 mL

【Notes】
a. 2×TaqMan qPCR Mix mainly contain heat-resistant UNICON® HotStart Taq DNA Polymerase, dNTPs, Mg2+, etc.
b. Prime&Probe Mix mainly contains target gene primer probes.
c. MPV Positive Control is enzymatic-free water.
d. MPV Positive Control is a plasmid containing the target gene and endogenous internal standard. The target gene is the FAM channel, and the concentration is 
1×104 copies/μL; the internal standard is the VIC channel.

Shipping and Storage

The product is shipped with ice pag and can be stored at -20°C for one year. This product avoids repeated freeze-thaw. It is recommended to save.

Reaction System(Take 25 μL for example)

Component	Volume	Final Concentration
2×TaqMan qPCR mix	12.5	1×
Primer&Probe mix	1	-
模板	5	-
ddH2O	up to 25	-

Applicable Models

ABI Series: ABI 5700, 7000, 7300, 7700, 7900HT Fast, StepOne, StepOne Plus; 7500, 7500 Fast, ViiA7, QuantStudio 3 and 5, QuantStudio 6, 7, 12k Flex;
Bio-Rad Series: Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4;
Roche Series: Roche Applied Science LightCycler 480, LightCycler 2.0; Lightcycler 96;
Others: Stratagene MX3000P, MX3005P, MX4000P;
Eppendorf Mastercycler ep realplex, realplex 2 s; 
Qiagen Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000;
Thermo Scientific PikoReal Cycler; Cepheid SmartCycler; Illumina Eco qPCR.

Standard Amplification Procedure

Stage	Temperature	Time	Cycles
Pre-digestion	37°C	5 min	1
Pre-denaturation	95°C	5 min	1
Amplification Reaction	95°C	15 sec	45
	60°C	30 sec

【Notes】:The annealing/extension temperature can be appropriately adjusted according to the experimental requirements.

Interpretation of Results

	FAM	Result
1	Ct＞38 or UNDET	Negative
2	Ct≤35	Positive
3	35<Ct≤38	Need to re-test, the Ct value is still in this range and the curve is in the standard "S" shape, it is judged as positive, otherwise it is judged as negative

Cautions

1.Please use the RNase free consumables during the experiment;
2.For your safety and health, please wear lab coats and disposable gloves for operation.