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mRNA synthesis process	Product name	Cat#
Template preparation	Hieff CanaceTM Plus High-Fidelity DNA Polymerase (Inquire)	10148ES
	Hieff CloneTM Universal One Step Cloning Kit	10922ES
	dNTP Mix (25 mM each)	10125ES
	FuniCut™ BsaI	15005ES
	FuniCut™ XbaI	15033ES
In vitro transcription	HifairTM T7 High Yield RNA Synthesis Kit	10623ES
	UCF.METM T7 RNA Polymerase GMP-grade（50 U/μL）(Inquire)	10624ES
	T7 RNA polymerase (50 U/μL) (Inquire)	10618ES
	NTP Set Solution (ATP, CTP, UTP, GTP, 100 mM each）	10133ES
	UCF.METM Pyrophosphatase,Inorganic GMP-grade	10620ES
	UCF.METM Murine RNase inhibitor GMP-grade	10621ES
Remove template DNA	UCF.METM Deoxyribonuclease I (DNase I) GMP-grade	10611ES
mRNA modification	UCF.METM mRNA Vaccinia Capping Enzyme GMP-grade	10614ES
	UCF.METM mRNA Cap 2´-O-Methyltransferase GMP-grade	10612ES
	GTP (100 mM)	10132ES
	S-adenosylmethionine (SAM)（32mM）	10619ES
mRNA purification	Hieff NGSTM RNA Cleaner	12602ES

African swine fever (ASF) is an acute, severe and highly contagious infectious disease caused by African swine fever virus (ASFV) infecting domestic pigs or wild boars. Its morbidity and mortality can reach 100%, seriously endangering the global pig industry and causing incalculable economic losses. The World Organization for Animal Health (OIE) lists it as a reportable animal disease, and my country lists it as a Class I animal disease.

 

 

At present, there is no vaccine or effective treatment for African swine fever. Rapid and accurate diagnosis of African swine fever through laboratory diagnostic methods is crucial to preventing the spread of the disease. Among laboratory detection technologies, qPCR is an important tool for routine diagnosis of African swine fever recommended by OIE, and it is also the preferred detection technology under the current situation of African swine fever.

 

 

Figure 1. Yeasen African Swine Fever related qPCR Products

 

 

Product advantages:

1. Efficient and convenient operation - Master Mix, no tedious operation, one-step sample addition

2. High detection sensitivity - genetically modified antibody method to hot-start Taq enzyme, 3 copies/reaction can be detected stably

3. stability of fully premixed PCR solution(Mix+Primers+Probes) - the working solution premixed can be stored at 37℃ for 7 days, and remains stable performance after repeated freezing and thawing

4. batch-to-batch stability - 20 repeated experiments of 4 different batches, CV<1%

5. High blood tolerance - can tolerate anticoagulant samples (EDTA, sodium citrate) and 0.5% whole blood

6. Suitable for multiple platforms - Bio-Rad CFX96, ABI Q5 and Slan are all compatible

 

 

 

ASFV detection solution 1: UDG fully premixed qPCR solution, super stability

 

 

Product collocation:
1.MolPureTM Magnetic Swab Viral DNA/RNA Kit（Cat#18300）
2.Hieff UniconTM  TaqMan Multiplex qPCR Master Mix（UDG plus）（Cat#13171）

 

 

Product advantages:

1.UDG anti-pollution system

2. Super stability of fully premixed PCR solution(Mix+Primers+Probes)

3. High amplification efficiency and good repeatability

4. Multi-platform multi-system applicable

 

 

 

Case show:

 

Super stability of fully premixed PCR solution

Cat#13171 was prepared into a fully premixed PCR solution containing primer and probes, placed at 37°C for 7 days, and repeated freezing and thawing 15 times to amplify the ASF plasmid system. The results showed that storage at 37°C for 7 days had no effect on the Ct value and sensitivity, and the Ct value did not change significantly after repeated freezing and thawing for 15 times.