Product Description

MicroRNAs are a class of non-coding RNAs with a length of about 22 nt, which play an important role in the regulation of gene expression in plants and animals. This kit uses Poly(A)-tailing method to perform reverse transcription from miRNA first-strand to cDNA. The 2×Hifair^TM miRNA RT buffer in the product contains all the raw materials and primers for miRNA Poly(A)-tailing reaction and reverse transcription reaction. Careful optimization ensures that Poly(A) modification process and reverse transcription process can be performed simultaneously and efficiently in miRNA 3' end.

This product is recommended to be used in conjunction with our company's Hieff^TM miRNA Universal qPCR SYBR Master Mix (Cat#11171) for optimal experimental results.

Shipping and Storage

The components are shipped with ice packs and stored at -20℃ for 1 year.

Cautions

1. Fully thaw the frozen components and mix gently before use.

2. It is recommended to amplify the fragment within 1 kb in length for the best amplification efficiency.

3. When experimenting, please use RNase-free consumables to avoid unnecessary losses affecting the experimental results.

4. Avoid repeated freezing and thawing of this product and avoid strong light exposure during preparation.

5. For your safety and health, please wear lab coats and disposable gloves for operation.

6. For research use only!