Description

Hieff™ Bst Plus DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I, lacking 5'-3' exonuclease activity. The enzyme has stronger 5'-3' DNA polymerase activity, strand displacement activity, and dUTP tolerance, which is more suitable for anti-pollution isothermal amplification reactions, such as LAMP, CPA, etc.

Features

• Thermophilic DNA polymerase with strong strand displacement activity

Applications

• Isothermal DNA amplification

Specifications

Polymerase	Bst DNA Polymerase
Heat Inactivation	Incubation at 85°C for 5 min.
Solution Components	10 mmol/L Tris-HCl, 50 mmol/L KCl, 0.1 mmol/L EDTA, 1 mmol/L DTT, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25℃.
Activity Definition	1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C.

Components

Components No.	Name	14402ES92 (8,000 U)	14402ES97 (40,000 U)
14402-A	Hieff™ Bst Plus DNA Polymerase (40 U/μL)	200 μL	1 mL
14402-B	10 ×Hieff™ Bst Plus DNA Polymerase Buffer	1 mL	3×1 mL
14402-C	100 mmol/L MgSO4	1 mL	3×1 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 2 years. Please avoid repeated freeze-thaw.