Product Description

Hieff NGS™ OnePot II DNA Library Prep Kit for MGI® developed for the MGI® platform utilize a patent non-restrictive endonuclease which fragments the DNA to targeted sizes in a time-dependent manner. This kit enables the single-step reaction of fragmentation, end-repair, and A-tailing without the requirement of beads clean-up, significantly reducing material loss, experimental costs, and hands-on time. The kit has a high library conversion rate and can be applied for most DNA samples from animals, plants, and microorganisms, including low-quality samples such as FFPE samples. By sequencing, all samples with different GC content can obtain excellent sequencing results, with high library coverage, good uniformity and low preference, which makes library construction simple and efficient. 

1. Compatible with 10 ng-1 μg DNA samples of most types, including cfDNA and FFPE samples

2. High-quality fragmentation enzyme which randomly fragments ds DNA without bias

3. Combining fragmentation, end-repair, and A-tailing in one single step

4. High fidelity polymerase with high amplification efficiency, significantly increasing library quality and yield

5. Compatible with FFPE DNA samples

6. Stringent quality control and batch effect elimination

Product Components

Components number and name			13321ES16	13321ES96
13321-A		Smearase™ Mix	160 μL	960 μL
13321-B		Ligation Enhancer	480 μL	4×720 μL
13321-C		Fast T4 DNA Ligase	80 μL	480 μL
13321-D		2×Ultima HF Amplification Mix	400 μL	4×600 μL
13321-E		Primer Mix for MGI®	80 μL	480 μL

Shipping and Storage

All the components are shipped with ice packs and can be stored at -20°C for one year.

Cautions

1 Operation

1.1 For your safety and health, please wear lab coats and disposable gloves for operation.

1.2 Please thaw each component of the kit at room temperature before use. Please invert the thawed reagents several times, briefly spin down, and put them on ice until use.

1.3 It is highly recommended to mix the reagents by pipetting up-and-down or by gentle vortexing when setting up the reactions. Vigorous vortexing may impact the library yield.

1.4 It is highly recommended to use filtered pipet tips to avoid cross-contamination. Be sure to change pipet tips when processing different samples.

1.5 It is highly recommended to pre-heat the lid of the thermocycler for each reaction step.

1.6 Improper operations may very likely cause carry-over contaminations through aerosols, impacting the experiment’s accuracy. It is highly recommended to divide the experiment environment into the pre-PCR and post-PCR regions, with separate sets of devices and disposables in each area. Perform routine cleaning for each area by wiping the surfaces with 0.5% sodium hypochlorite or 10% bleach.

1.7 For research use only!