Product Description

The Hieff NGS Dual Barcode Fast-Pace DNA Cyclization Kit for MGI is a single-strand cyclization kit specifically designed for MGI high-throughput sequencing platforms. Using this kit, the ligated MGI-double Barcode standard PCR products can be prepared into single-stranded circular DNA libraries suitable for MGI high-throughput sequencers.

Product Components

Components number and name			13340ES16	13340ES96
13340-A		DB Splint Oligo	96 μL	576 μL
13340-B		Splint Buffer	240 μL	2×720 μL
13340-C		Ligase	80 μL	480 μL
13340-D		Digestion Buffer	128 μL	768 μL
13340-E		Digestion Enzyme	32 μL	192 μL

Shipping and Storage

All the components are shipped with dry ice and can be stored at -20°C for one year.

Cautions

Ⅰ About the operation

1. For your safety and health, please wear lab coats and disposable gloves for operation.

2. Please thaw each component of the kit at room temperature before use. Please invert the thawed reagents several times, briefly spin down, and put them on ice until use.

3. It is highly recommended to mix the reagents by pipetting up-and-down or by gentle vortexing when setting up the reactions. Vigorous vortexing may impact the library yield.

4. It is highly recommended to use filtered pipet tips to avoid cross-contamination. Be sure to change pipet tips when processing different samples.

5. It is highly recommended to pre-heat the lid of the thermocycler for each reaction step.

6. Improper operations may very likely cause carry-over contaminations through aerosols, impacting the experiment’s accuracy. It is highly recommended to divide the experiment environment into the pre-PCR and post-PCR regions, with separate sets of devices and disposables in each area. Perform routine cleaning for each area by wiping the surfaces with 0.5% sodium hypochlorite or 10% bleach.

7. For research use only！