Product Description

Hieff NGS™MaxUp rRNA Depletion Kit (Plant) is designed to remove rRNA (including cytoplasmic 18S and 25S rRNA, mitochondrial 18S and 26S rRNA, chloroplast 16S and 23S rRNA) from plant total RNA based on RNase H-based workflow and to retain mRNA and other non-coding RNA. This kit is suitable for both intact and partially degraded RNA samples.

Product Application

This kit can be used for 100 ng-1 μg total RNA samples from various plants. The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis.

Shipping and Storage

All the components are shipped with dry ice and can be stored at -20°C for one year.

Required Materials Not Included

1. RNA clean beads: Hieff NGS™Cleaner (Cat#12602) or equivalent.

2. qRT-PCR inspection for rRNA removal efficiency detection: Hieff™qPCR SYBR Green Master Mix (No Rox) (Cat#11201) or equivalent. 

3. Other materials: Ethanol, ddH2O, Pipettes, PCR tubes, Magnetic stand, Thermocycler.

Cautions

1. Please use consumables that are free of RNase contamination and clean the experimental area regularly. It is recommended to use ThermoFisher's RNAZap™ high-efficiency nucleic acid removal spray to remove RNase contamination.

2. The RNA sample should be free from genomic DNA contamination. If gDNA remains in the sample, it should be digested by DNase I and purified before use. 

3. The maximum input volume of RNA sample is 10 μL. If the sample volume is large, it can be concentrated first. 

4. For your safety and health, please wear lab coats and disposable gloves for operation.

5. For research use only!