Product Description

Hieff NGS™ Fast-Pace DNA Cyclization Kit for MGI® is a single-strand cyclization kit specifically designed for MGI® high-throughput sequencing platforms. The use of high-quality enzymes and optimized Buffer significantly improves reaction efficiency, enabling the entire cyclization and digestion process to be completed in less than 30 minutes. This kit is suitable for all standard dual-label PCR adapter library connected to MGI® platforms and is not limited to different MGI sequencing platforms except for the limitations of the library-prep reagents.

Product Components

Components number and name			13341ES16	13341ES96
13341-A		Splint Oligo	96 μL	576 μL
13341-B		Splint Buffer	240 μL	2×720 μL
13341-C		Ligase	80 μL	480 μL
13341-D		Digestion Buffer	128 μL	768 μL
13341-E		Digestion Enzyme	32 μL	192 μL

Shipping and Storage

All the components are shipped with ice packs and can be stored at -20°C for one year.

Cautions

Ⅰ About the operation

1. For your safety and health, please wear lab coats and disposable gloves for operation.

2. Please thaw each component of the kit at room temperature before use. Please invert the thawed reagents several times, briefly spin down, and put them on ice until use.

3. It is highly recommended to mix the reagents by pipetting up-and-down or by gentle vortexing when setting up the reactions. Vigorous vortexing may impact the library yield.

4. It is highly recommended to use filtered pipet tips to avoid cross-contamination. Be sure to change pipet tips when processing different samples.

5. It is highly recommended to pre-heat the lid of the thermocycler for each reaction step.

6. Improper operations may very likely cause carry-over contaminations through aerosols, impacting the experiment accuracy. It is highly recommended to divide the experiment environment into the pre-PCR and post-PCR regions, with separate sets of devices and disposables in each area. Perform routine cleaning for each area by wiping the surfaces with 0.5% sodium hypochlorite or 10% bleach.

7. For research use only!