This kit adopts efficient magnetic beads, combined with a unique buffer system, which can specifically bind RNA and effectively remove proteins, salt ions and other impurities. It is often used to purify total RNA samples after rRNA removal, in vitro transcribed RNA products, RNA-labeled products, and synthetic RNAs. And the purified RNA is suitable for RNA library construction, RT-PCR, qRT-PCR, chip analysis, Northern Blot and RNAi experiments.
The beads are shipping with ice packs and can be stored at 2-8°C for one year. Avoid freezing!
Changes in qRT-PCR Ct values of RNA samples before and after using the total RNA purification kit.
RNA Sample |
Human |
Mouse |
Arabidopsis |
|||
Detect genes |
GAPDH |
β-Actin |
GAPDH |
β-Actin |
PP2A |
TUB2 |
Ct before purification |
12.92 |
12.36 |
18.74 |
18.53 |
24.33 |
22.2 |
Ct after purification |
12.32 |
11.89 |
17.62 |
17.91 |
23.38 |
21.35 |
[Note]: Using Hieff NGS™ RNA Cleaner can effectively remove inhibitorsof RT-PCR, making the results of the experiments more accurate.
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